The backbone amide nitrogen sequence is extracted from a PDB file using the same commands as the relaxation curve-fitting script (Chapter 5. The command
[firstnumber=6] # Load the sequence from a PDB file. structure.read_pdb('Ap4Aase_new_3.pdb')
will load the PDB file Ap4Aase_new_3.pdb into relax. Then the following commands will generate both the backbone amide and tryptophan indole 15N spins
[firstnumber=8] structure.load_spins(spin_id='@N') structure.load_spins(spin_id='@NE1')