Hello Users,
I am new to the world of model-free analysis, I have my first data set of
R1, R2, and NOE at 800mHz, at 35C and 50C. I would like to use relax as the
program for analysis, as it seems both robust and user-friendly.
Unfortunately, it would be quite difficult for me to acquire data at a
lower field since my protein is ~40kDa and the fact that the 600mHZ
spectrometers at my university can only decouple, but not detect, 15N.
I am curious, what catastrophic event would occur if I were to try to use
relax with only data sets from 800mHz? I've read through the references,
but could someone make it abundantly clear to me why this is a horrible
idea? Otherwise, I will have to use modelfree, and after reading through
the d'Auvergne/Gooley 2008 J Biomol NMR papers, I feel like that won't be
so great either.
Thank you,
Sean Moro
PhD candidate
University of California, Irvine.
What happens if I try to do MF analysis only having 800mHz data?