Hi Sam.
Try to have a look on the wiki:
http://wiki.nmr-relax.com/Category:Tutorials
http://wiki.nmr-relax.com/Tutorial_for_Relaxation_dispersion_analysis_cpmg_fixed_time_recorded_on_varian_as_fid_interleaved
Maybe you can figure out something.
If you need more help.
Make a suppert request
https://gna.org/support/?group=relax
Upload an example peak list for each CPMG frq.
Make a file, listing filename for peaklist and corresponding CPMG frq.
You need to determine the error of your experiments.
If you have peak heights which are replicate/triplicate, then this is fine.
Or else you need to measure RMSD of the noise in the spectrum.
What is also:
Relaxation dispersion CPMG constant time delay T (in s).
The NMR field strength of the spectrum. (750 MHz ?)
Best
Troels
2015-02-15 1:09 GMT+01:00 Sze Chan <samuelsw.chan@xxxxxxxxxxxxxxxx>:
Hello all,
I am an undergrad that is new to the field of NMR and I was hoping to get
some basic help in setting up my data for analysis.
For my CPMG relaxation dispersion experiments, I usually process my data
through NMR pipe and then extract the peak heights through ccpnmr with each
plane as the CPMG frequency. In the end for each residue, I get the peak
height at various CPMG frequencies.
However, I'm not sure how to format my table or know what software I can
use to format my data so that I can input it into relax. If anyone has any
help, I'd greatly appreciate it since I'm very new to the field and all the
analysis that comes with it.
Regards,
Sam
Department of Biochemistry - University of Toronto
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