Re: Relax_fit.py problem -- October 15, 2008 - 22:03

The random coil region is the area around 8 ppm (+/- 0.5 ppm) in the
proton dimension where the protons of protein backbone amides located
in coils, rather than ordered alpha or beta secondary structure, lye.
Are your 1D experiments of proteins, RNA, DNA, or small molecules?  I
would guess that this effect will occur anywhere where there are many
intense signals, such as a solvent signal.

Regards,

Edward


On Wed, Oct 15, 2008 at 9:42 PM, Tyler Reddy <TREDDY@xxxxxx> wrote:
> Also, what is this 'random coil' region of an NMR spectrum? Are you
> referring to
> areas of higher noise like near the water peak or between peaks versus a
> 100%
> noise region of the spectrum?
>
>
> > This would save NMR time and it would be sufficient for a model-free
> > analysis if done carefully.  Remember that the RMSD value in the
> > random coil region is higher than the empty parts of the spectrum, so
> > don't forget to get some estimates by measuring in there.
> >
> > Regards,
> >
> > Edward