Dear relax-users,
I am just beginning to record and analyze relaxation data for MF analysis. So
far we collected data at 600 MHz, and a second data set at 750MHz is planned.
I have two questions I hope you can help me with:
1) Temperature calibration
We use single-scan interleaving to account for a constant temperature during
the whole experimental series. We also use methanol to calibrate the
spectrometer's temperature sensors. As we found out, every magnet is
different and the on-screen parameters cannot be trusted. We have tables
where This procedure is not done with the T2 measurements in mind, however.
Isn't it also important that R1 and R2 measurements have the same temperature
– how do you check for that?
How can I check differences in sample heating between different magnets due
to different air-flow etc? How to compensate for these temperature
differences? Do I need different pulse programs that account for temperature
differences for every spectrometer? Or is the difference insignificant once
the spectrometers have been calibrated?
2)
In Mac OS X ("Lion", 10.7.3), the self-contained relax Application bundle is
crashing and is giving just an "relax error" and the option to "terminate" or
"open the Console".
I can run the “relax” script which is sitting inside
relax.app/Contents/Resources/ and I get a prompt, but only after installing
numpy via Macports. If I don't, relax complains that numpy is not installed.
After installing wxPython 2.9 via the official installer "./relax" -g is
attempting to start (I can see the splash screen), but then halts with the
errors.
Interestingly, I found out today that it seems to run just fine if I run the
relax binary in Contents/MacOS by dropping it onto Terminal.app!
(I did not test this without numpy and wXPython seperately installed.)
What's your experience? What do you think is broken – my system (10.7) or
something inside the application bundle?
Cheers
Martin
***
RELAX ERROR MESSAGES FROM THE CONSOLE
15.03.12 15:19:15,538 relax: relax Error
15.03.12 15:19:17,667 com.apple.launchd.peruser.501:
([0x0-0x14e14e].com.nmr-relax.relax[70595]) Exited with code: 255
15.03.12 15:19:22,227 [0x0-0x14f14f].com.nmr-relax.relax: <_FuncPtr object at
0x101b0dc80>
15.03.12 15:19:22,227 [0x0-0x14f14f].com.nmr-relax.relax: Traceback (most
recent call last):
15.03.12 15:19:22,227 [0x0-0x14f14f].com.nmr-relax.relax: File
"/Applications/relax.app/Contents/Resources/__boot__.py", line 179, in
<module>
15.03.12 15:19:22,227 [0x0-0x14f14f].com.nmr-relax.relax:
_argv_emulation()
15.03.12 15:19:22,227 [0x0-0x14f14f].com.nmr-relax.relax: File
"/Applications/relax.app/Contents/Resources/__boot__.py", line 177, in
_argv_emulation
15.03.12 15:19:22,227 [0x0-0x14f14f].com.nmr-relax.relax:
_get_argvemulator().mainloop()
15.03.12 15:19:22,227 [0x0-0x14f14f].com.nmr-relax.relax: File
"/Applications/relax.app/Contents/Resources/__boot__.py", line 101, in
mainloop
15.03.12 15:19:22,227 [0x0-0x14f14f].com.nmr-relax.relax: stoptime =
Evt.TickCount() + timeout
15.03.12 15:19:22,227 [0x0-0x14f14f].com.nmr-relax.relax: AttributeError:
'module' object has no attribute 'TickCount'
--
Martin Ballaschk
AG Schmieder
Leibniz-Institut für Molekulare Pharmakologie
Robert-Rössle-Str. 10
13125 Berlin
ballaschk@xxxxxxxxxxxxx
Tel.: +49-30-94793-234/315
Büro: A 1.26
Labor: C 1.10
Temperature calibration & relax @ Mac OS X 10.7.3 „Lion“