Hi Troels,
The -fn REV part is needed for certain acquisition modes. I copied
the script contents for one such spectrum. This option can be removed
for most 2D experiments though. Looking closely at the script I have
noticed that there are a few things wrong with it or at least not
ideal for a relaxation analysis. For example the extract function
should be performed at the very end. In this case it does not matter
as a polynomial baseline correction is not performed in the indirect
dimension but, if it were to be added, performing the data trimming
after a polynomial correction results in a better baseline. I must
have copied the wrong script. I'll look into this and change the
script contents to one of the scripts I use for processing spectra for
standard R1, R2 or NOE spectra from back in my PhD days.
Cheers,
Edward
On 25 August 2013 18:45, Troels Emtekær Linnet <tlinnet@xxxxxxxxx> wrote:
Hi.
I was looking the in the manual, at page: 52
5.2.2 Spectral processing
There is an example of processing script for NMRPipe.
It mentions the line:
| nmrPipe -fn REV \
which is for reversing the spectrum, since the CPMG element in a pulse
sequence will make the magnetization end up negative.
According to the manual:
http://spin.niddk.nih.gov/NMRPipe/ref/nmrpipe/rev.html
The recommendation is to do a negative Fourier Transform
| nmrPipe -fn FT -neg \
Is there a special reason for | nmrPipe -fn REV \
Best
Troels Emtekær Linnet
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Re: Using "| nmrPipe -fn REV \" for processing CPMG dispersiong experiments