Hello everyone, This isn't as much a problem with relax as it is a general question regarding S^2 values itself. When comparing different values of S^2 between proteins, what difference is considered significant. Due to the lower values I know say an average S^2 value of .1 is a big difference in the flexibility of one protein to another, but what about say an average difference of .01, is that still considered a significant change in considering the relative dynamics of one protein to another? (I.e. if say, protein A has an average S^2 value of 9, and protein B has an average S^2 value of 8.9, could you make the statement Protein A has significant dynamic differences, more rigid, when compared to Protein B. Or could you make the statement due to only a difference of .01, there isn't much of a overall dynamic difference between the 2 proteins)? Sincerely, Sam