Hi everyone I got a question concerning the treatment of Rex in reduced spectral density mapping as implemented in relax... My data include R1, R2 and {1H}15N-NOE at three magnetic fields (500, 600 and 800 MHz). When proceeding to the reduced spectral density mapping, I obtain J at three values (0, wN and wH). However, a slight trend is observable between those extracted values, i.e. the values from data at 500 MHz are higher than those at 600, which are higher than those at 800... Is it normal ? Is there a way to extract the contribution from Rex (conformational exchange, slow us-ms timescale motions) from my data using reduced spectral density mapping at those three fields ? Thanks !! Sébastien :) -- ______________________________________ _______________________________________________ | | || Sebastien Morin || ||| Etudiant au PhD en biochimie ||| |||| Laboratoire de resonance magnetique nucleaire |||| ||||| Dr Stephane Gagne ||||| |||| CREFSIP (Universite Laval, Quebec, CANADA) |||| ||| 1-418-656-2131 #4530 ||| || || |_______________________________________________| ______________________________________