Hi Sébastien,
The field dependence you note is expected for J(wN) and J(wH), because
the frequencies wN and wH are of course field dependent, and J(w)
decreases with increasing frequency. Field dependence in J(0) is not
expected, however. In fact I often use the consistency of J(0) across
datasets as a check on the quality of the data - any variation in J(0)
across datasets, irrespective of field, is an indication of
inconsistencies in the data.
If you are seeing inconsistency in J(0), then there are any number of
possible problems that might contribute. Changes in sample temperature
in the various magnets and sample instability (aggregation or
proteolytic degredation, eg) both tend to affect J(0) by changing the
apparent rotational diffusion of the molecule; imperfect water
supression causes errors in relaxation measurements, etc ...
Hope this helps,
Chris
On Tue, 2006-12-12 at 11:34 -0500, Sebastien Morin wrote:
Hi everyone
I got a question concerning the treatment of Rex in reduced spectral
density mapping as implemented in relax...
My data include R1, R2 and {1H}15N-NOE at three magnetic fields (500,
600 and 800 MHz). When proceeding to the reduced spectral density
mapping, I obtain J at three values (0, wN and wH). However, a slight
trend is observable between those extracted values, i.e. the values from
data at 500 MHz are higher than those at 600, which are higher than
those at 800...
Is it normal ?
Is there a way to extract the contribution from Rex (conformational
exchange, slow us-ms timescale motions) from my data using reduced
spectral density mapping at those three fields ?
Thanks !!
Sébastien :)
Re: Reduced spectral density mapping VS Rex