15N data at 1 field, 2H data -- March 18, 2008 - 13:02

Dear relax users:

I have a couple of questions regarding the relax program.

First, the example scripts for model-free selection and optimization described in the relax manual utilize 15N relaxation data recorded at two or three fields. Does anyone have advice/experience in using relax to fit T1, T2 and NOE data recorded at only one field, particularly to models that include anisotropic global motion?

Idealy, I would like to follow a local t_m approach to model-free model selection and optimization, as recently described [d'Auvergne and Gooley (2008) J. Biomol. NMR 40, 107-119; ibid., 121-133], rather than a "diffusion seeded" approach. With data at one field, this analysis would be limted to models tm0 through tm3, and tm9. Are there relax scripts available for this type of an analysis with data at one field? Also, again using data from only one field, could one readily discern from the results of a local t_m analysis if isotropic diffusion models are adequate? If adequate, this would allow fitting the data to models m0 through m5, and m9.

A final question - have any facilites been added to relax (or planned) for fitting methyl ²H relaxation data?

Thank you very much for your consideration. Any suggestions would be greatly appreciated.

      Keith Constantine

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