Hi Edward,
a part of my mail is missing, it seems like I accidentally sent a draft
version.
Although it's maybe not that important, here is the rest of the final
paragraph:
„I don't know if inaccurate or inconsistent data would favor such a behavior.
We now use selective proton pulses in the R1 and NOE-experiments (like
described in [1]), temperature compensation for our R1 pulse programs,
single-scan interleaving for all experiments, accurate temperature
calibration with d4-methanol and automated and hence reproducable peak
picking and fitting routines, and recycle delays of 10s in the HetNOE. The
consistency of the data from two fields was well below 8% even when the
temperature was (accidentally) off by 1K.“
Regards,
Martin
[1] Lakomek N-A, Ying J, Bax A (2012) Measurement of 15N relaxation rates in
perdeuterated proteins by TROSY-based methods. J Biomol NMR 53: 209–221.
doi:10.1007/s10858-012-9626-5.
--
Martin Ballaschk
AG Schmieder
Leibniz-Institut für Molekulare Pharmakologie
Robert-Rössle-Str. 10
13125 Berlin
ballaschk@xxxxxxxxxxxxx
Tel.: +49-30-94793-234/315
Büro: A 1.26
Labor: C 1.10
Re: m0 models