unexpected mf S2 valuesunexpected mf S2 values|
Hi,
I've conducted model-free analysis using the default protocol set up in the GUI on a protein for which I have data recorded at 600 and 800 MHz. The order parameters in s2.txt look very strange: regions I know are dynamic have larger S2 values than those
in the "structured" regions. My understanding is that if the value is close to zero then it is highly dynamic and if close to 1 => very rigid. My results are the inverse of what I'm expecting. The calculation ran without any errors and I've checked the input
data for any obvious errors. Has anyone come across this before?
Another strange thing is that the regions I know to be structured (which seem to have small S2 values) have very large associated errors. The N- and C-termini of my protein however have higher S2 values but have smaller associated errors.
Does any one know what could have happened?
Cheers,
Soumya
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